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breast epithelial cell lines mcf10a  (ATCC)


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    Structured Review

    ATCC breast epithelial cell lines mcf10a
    Breast Epithelial Cell Lines Mcf10a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epithelial+cell+line+mcf10a/MCF+10A/pm42210419-64-10-18
    Average 99 stars, based on 8125 article reviews
    breast epithelial cell lines mcf10a - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: Evaluating the Antiproliferative Effects of Tri(2-Furyl)- and Triphenylphosphine-Gold(I) Pyridyl- and Pyrimidine-Thiolate Complexes
    Article Snippet: In the first phase, we investigated the cytotoxic effects of Series 1 and 2, as well as auranofin and cisplatin controls, against the SK-BR-3 (ATCC HTB-30) breast cancer cell line. .. In the second phase, we identified a top candidate from each series and measured half-maximal effective concentrations (EC 50 ) against the malignant mammary cell lines MCF7 (ATCC HTB-22) and MDA-MB-231 (ATCC HTB-26), as well as the mammary epithelial cell line MCF10A (ATCC CRL-10317) and the non-transformed fibroblast cell line HEK293T (ATCC CRL-11268). ..

    Article Title: TCF12-regulated GRB7 facilitates the HER2+ breast cancer progression by activating Notch1 signaling pathway.
    Article Snippet: .. All human BC cell lines including ER+ BC cell lines MCF7 and T47D, HER2+ BC cell lines SK-BR-3, MDAMB-453, and TNBC cell lines MDA-MB-231 and MDAMB-468 and the immortalized mammary epithelial cell line MCF10A were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). ..

    Article Title: Has_circ_0048764 promotes breast cancer progression by sponging miR-578 and regulating HMGA2 expression.
    Article Snippet: Background: Circular RNAs (circRNAs) function as important regulators in the progression of cancers.. The role of circRNA_0048764 (circ_0048764) in the development of breast cancer (BC) remains inconclusive.. This work investigates the biological function and molecular mechanism of circ_0048764 in BC.

    Article Title: TCF12-regulated GRB7 facilitates the HER2+ breast cancer progression by activating Notch1 signaling pathway
    Article Snippet: .. All human BC cell lines including ER+ BC cell lines MCF7 and T47D, HER2+ BC cell lines SK-BR-3, MDA-MB-453, and TNBC cell lines MDA-MB-231 and MDA-MB-468 and the immortalized mammary epithelial cell line MCF10A were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). ..

    Cell Culture:

    Article Title: GAS5 regulated by FTO-mediated m6A modification suppresses cell proliferation via the IGF2BP2/QKI axis in breast cancer
    Article Snippet: The human breast cancer cell lines BT-474, SKBR3, BT-20, MDA-MB-231, MCF-7, and T-47D were obtained from American Type Culture Collection (ATCC). .. Cells were cultured in Dulbecco’s modified eagle medium (DMEM) (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% streptomycin-penicillin in a humidified atmosphere at 37 °C with 5% CO 2 .The immortalized mammary epithelial cell line MCF10A were obtained from ATCC and cells were cultured in Dulbecco’s Modified Eagle Medium: F-12(DMEM/F-12) (Gibco, USA) supplemented with 5% horse serum, 50 ng/ml epidermal growth factor (EGF), 10 mg/ml insulin, 0.5 mg/ml hydrocortisone, 0.1 mg/ml cholera toxin and 1% streptomycin-penicillin at 37 °C in an atmosphere of 5% CO2 and 95% air. .. The full-length fragments of GAS5, FTO, and IGF2BP2 were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) and subcloned into the pcDNA3.1 vector, named GAS5-OE, FTO-OE, and IGF2BP2-OE.

    Modification:

    Article Title: GAS5 regulated by FTO-mediated m6A modification suppresses cell proliferation via the IGF2BP2/QKI axis in breast cancer
    Article Snippet: The human breast cancer cell lines BT-474, SKBR3, BT-20, MDA-MB-231, MCF-7, and T-47D were obtained from American Type Culture Collection (ATCC). .. Cells were cultured in Dulbecco’s modified eagle medium (DMEM) (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% streptomycin-penicillin in a humidified atmosphere at 37 °C with 5% CO 2 .The immortalized mammary epithelial cell line MCF10A were obtained from ATCC and cells were cultured in Dulbecco’s Modified Eagle Medium: F-12(DMEM/F-12) (Gibco, USA) supplemented with 5% horse serum, 50 ng/ml epidermal growth factor (EGF), 10 mg/ml insulin, 0.5 mg/ml hydrocortisone, 0.1 mg/ml cholera toxin and 1% streptomycin-penicillin at 37 °C in an atmosphere of 5% CO2 and 95% air. .. The full-length fragments of GAS5, FTO, and IGF2BP2 were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) and subcloned into the pcDNA3.1 vector, named GAS5-OE, FTO-OE, and IGF2BP2-OE.



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    ATCC mammary epithelial cell line mcf10a
    (A) Dose–response curves of Ga3 and Ga6 compared to cisplatin in nontransformed cell lines HaCaT and <t>MCF10A.</t> (B) Selectivity Index values for Ga3 , Ga6 and cisplatin. Data represent the mean ± SD from three independent experiments performed in triplicate.
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    (A) Dose–response curves of Ga3 and Ga6 compared to cisplatin in nontransformed cell lines HaCaT and MCF10A. (B) Selectivity Index values for Ga3 , Ga6 and cisplatin. Data represent the mean ± SD from three independent experiments performed in triplicate.

    Journal: Journal of Medicinal Chemistry

    Article Title: Exploring Gallium(III) Complexes as Emerging Therapeutic Candidates for Breast Cancer

    doi: 10.1021/acs.jmedchem.5c03480

    Figure Lengend Snippet: (A) Dose–response curves of Ga3 and Ga6 compared to cisplatin in nontransformed cell lines HaCaT and MCF10A. (B) Selectivity Index values for Ga3 , Ga6 and cisplatin. Data represent the mean ± SD from three independent experiments performed in triplicate.

    Article Snippet: The cell lines MCF7, SKBR3, BT549, and MDA-MB-231, the immortalized nontransformed keratinocyte cell line HaCaT, and the human mammary epithelial cell line MCF10A were acquired from ATCC.

    Techniques:

    Matrigel assay. (A) 3D structures of MCF7, MDA-MB-231 and MCF10A seeded in Matrigel matrix and treated with compound at indicated doses for 72-h. Scale bar = 250 μm. (B) The diameter of 3D structures was quantified using ImageJ. Data are the average ± SEM of three independent experiments. The values for the statistical analyses are * p ≤ 0.05; *** p ≤ 0.001.

    Journal: Journal of Medicinal Chemistry

    Article Title: Exploring Gallium(III) Complexes as Emerging Therapeutic Candidates for Breast Cancer

    doi: 10.1021/acs.jmedchem.5c03480

    Figure Lengend Snippet: Matrigel assay. (A) 3D structures of MCF7, MDA-MB-231 and MCF10A seeded in Matrigel matrix and treated with compound at indicated doses for 72-h. Scale bar = 250 μm. (B) The diameter of 3D structures was quantified using ImageJ. Data are the average ± SEM of three independent experiments. The values for the statistical analyses are * p ≤ 0.05; *** p ≤ 0.001.

    Article Snippet: The cell lines MCF7, SKBR3, BT549, and MDA-MB-231, the immortalized nontransformed keratinocyte cell line HaCaT, and the human mammary epithelial cell line MCF10A were acquired from ATCC.

    Techniques: Matrigel Assay